The Human FTH (Ferritin Heavy Polypeptide) CLIA Kit is a cutting-edge assay designed for the precise measurement of FTH levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results that are essential for a variety of research purposes.FTH is a critical protein involved in iron storage and transport within the body. Dysregulation of FTH levels has been linked to various diseases, including anemia, iron overload disorders, and inflammatory conditions.
Studying FTH levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of potential diagnostic and therapeutic strategies.Whether you are investigating iron metabolism disorders, inflammatory diseases, or other related research areas, the Human FTH CLIA Kit provides a reliable tool for measuring FTH levels with precision and efficiency. Stay ahead in your research with this advanced assay kit from AssayGenie.
Product Name:
Human FTH (Ferritin, Heavy Polypeptide) CLIA Kit
SKU:
HUES01087
Target:
Human FTH (Ferritin, Heavy Polypeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FTH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FTH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FTH. You can calculate the concentration of Human FTH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
86-98
89-104
Average (%)
100
91
95
1:4
Range (%)
96-110
93-107
88-100
Average (%)
102
100
94
1:8
Range (%)
88-104
98-110
95-112
Average (%)
96
104
102
1:16
Range (%)
85-96
94-108
99-112
Average (%)
90
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.46
7.86
0.98
2.29
8.16
Standard deviation
0.08
0.21
0.66
0.1
0.22
0.79
C V (%)
8.7
8.54
8.4
10.2
9.61
9.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FTH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FTH in samples. No significant cross-reactivity or interference between Human FTH and analogues was observed.
