Human G-CSF (Granulocyte Colony Stimulating Factor 3) CLIA Kit
The Human G-CSF (Granulocyte Colony-Stimulating Factor) CLIA Kit is specifically designed for the accurate measurement of G-CSF levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.G-CSF is a key cytokine that stimulates the production of granulocytes (a type of white blood cell) in the body. It plays a crucial role in immune response and has been implicated in various diseases and disorders, including leukemia, infections, and autoimmune conditions.
By accurately measuring G-CSF levels, researchers can gain valuable insights into the role of this cytokine in different pathological conditions and potentially identify new therapeutic targets.Overall, the Human G-CSF CLIA Kit from Assay Genie provides a reliable tool for studying the role of G-CSF in health and disease, offering researchers a valuable resource for advancing their research in the field of immunology and beyond.
Product Name:
Human G-CSF (Granulocyte Colony Stimulating Factor 3) CLIA Kit
SKU:
HUES00077
Target:
Human G-CSF (Granulocyte Colony Stimulating Factor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human G-CSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human G-CSF. You can calculate the concentration of Human G-CSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
84-95
98-109
Average (%)
96
90
103
1:4
Range (%)
99-112
92-106
95-108
Average (%)
106
98
100
1:8
Range (%)
96-110
96-114
84-98
Average (%)
102
104
91
1:16
Range (%)
86-100
90-104
98-110
Average (%)
92
96
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
93-108
98
Cell culture media (n=5)
83-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.35
101.99
383.49
37.71
104.98
353.05
Standard deviation
4.32
10.75
33.98
3.16
10.6
30.61
C V (%)
11.57
10.54
8.86
8.38
10.1
8.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human G-CSF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human G-CSF in samples. No significant cross-reactivity or interference between Human G-CSF and analogues was observed.
