The Human GAD2 (Glutamate Decarboxylase 2) ELISA Kit is expertly crafted to facilitate the precise and quantitative detection of GAD2 protein levels in various human biological samples. GAD2, a key enzyme involved in amino acid metabolism, plays a pivotal role in catalyzing the conversion of glutamate to gamma-aminobutyric acid (GABA). This conversion is essential for inhibitory neurotransmission in the central nervous system and is integral to maintaining the balance of excitatory and inhibitory signaling. Accurate quantification of GAD2 levels using our ELISA kit offers valuable insights into neurological function, neurotransmitter balance, and neurodegenerative conditions.
With exceptional sensitivity and specificity, our ELISA kit ensures dependable and reproducible results. Engineered under stringent quality standards, this kit delivers robust performance and simplicity, making it suitable for both research and clinical investigations. Place your trust in our Human GAD2 ELISA Kit for reliable and precise measurement of this critical enzyme in your research endeavors.
Product Name:
Human GAD2 (Glutamate Decarboxylase 2) ELISA Kit
SKU:
AEES00105
Target:
Human GAD2 (Glutamate Decarboxylase 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAD2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAD2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAD2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAD2. You can calculate the concentration of Human GAD2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
87-95
98-107
Average (%)
96
90
102
1:4
Range (%)
96-108
95-106
93-100
Average (%)
104
102
97
1:8
Range (%)
97-105
88-97
86-100
Average (%)
100
92
94
1:16
Range (%)
95-109
86-94
87-99
Average (%)
103
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
97
EDTA plasma (n=5)
101-107
104
Cell culture media (n=5)
87-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.71
556.55
2120.82
232.74
603.91
2042.33
Standard deviation
11.32
26.1
104.13
12.75
28.2
88.02
C V (%)
5.4
4.69
4.91
5.48
4.67
4.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GAD2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAD2 in samples. No significant cross-reactivity or interference between Human GAD2 and analogues was observed.
