The Human Galanin ELISA Kit is a powerful tool for researchers looking to accurately measure galanin levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it essential for a variety of research applications.Galanin is a neuropeptide that plays a key role in various physiological processes, including pain modulation, stress response, and appetite regulation.
Dysregulation of galanin has been linked to neurological disorders, mood disorders, and chronic pain conditions, highlighting its importance as a biomarker for understanding and potentially treating these conditions.By utilizing the Human Galanin ELISA Kit, researchers can gain valuable insights into galanin levels in different biological samples, providing valuable data for studying the role of galanin in health and disease.
Product Name:
Human GAL (Galanin) ELISA Kit
SKU:
HUES02357
Target:
Human GAL (Galanin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAL. You can calculate the concentration of Human GAL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
86-101
90-106
Average (%)
101
94
97
1:4
Range (%)
92-105
81-91
88-101
Average (%)
97
87
93
1:8
Range (%)
94-107
86-101
84-99
Average (%)
100
92
91
1:16
Range (%)
92-102
79-92
80-92
Average (%)
97
85
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.57
88.89
395.04
47.41
86.81
431.66
Standard deviation
2.94
3.78
15.21
2.81
4.82
14.03
C V (%)
5.93
4.25
3.85
5.93
5.55
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GAL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL in samples. No significant cross-reactivity or interference between Human GAL and analogues was observed.
