The Human Gal-2 (Galectin-2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Galectin-2 levels in human samples such as serum, plasma, and cell culture supernatants. Galectin-2 is a protein involved in various biological processes, including inflammation, immune response, and cell adhesion.This kit provides researchers with reliable and reproducible results, making it ideal for a wide range of research applications related to studying Galectin-2's role in various diseases and conditions.
Galectin-2 is known to be associated with inflammatory diseases, autoimmune disorders, and cancer, making it a valuable biomarker for understanding disease pathogenesis and potential therapeutic interventions.Overall, the Human Gal-2 CLIA Kit from Assaygenie offers researchers a powerful tool for accurately measuring Galectin-2 levels in human samples, enabling further exploration of its biological functions and potential implications in various disease states.
Product Name:
Human GAL2 (Galectin 2) CLIA Kit
SKU:
HUES00619
Target:
Human GAL2 (Galectin 2 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL2. You can calculate the concentration of Human GAL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
92-106
85-96
Average (%)
108
99
91
1:4
Range (%)
89-105
97-112
101-117
Average (%)
96
105
109
1:8
Range (%)
99-116
90-102
92-107
Average (%)
106
95
99
1:16
Range (%)
91-106
98-115
86-99
Average (%)
97
105
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
101-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.94
612.78
1710.78
214.91
672.61
1782.57
Standard deviation
18.1
68.57
133.95
24.07
79.91
178.61
C V (%)
8.92
11.19
7.83
11.2
11.88
10.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL2 in samples. No significant cross-reactivity or interference between Human GAL2 and analogues was observed.
