The Human Gal4-Galectin-4 CLIA Kit is specifically designed for the precise measurement of Gal4-Galectin-4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Gal4-Galectin-4 is a key protein involved in cell adhesion and signaling processes, playing a critical role in various physiological and pathological conditions. Its dysregulation has been linked to disease states such as cancer, inflammatory disorders, and autoimmune diseases, making it a valuable biomarker for understanding disease mechanisms and exploring potential therapeutic interventions.
The Human Gal4-Galectin-4 CLIA Kit from Assay Genie offers researchers a reliable tool for studying the role of Gal4-Galectin-4 in health and disease, providing valuable insights into its molecular function and potential clinical implications. Upgrade your research capabilities with this innovative assay kit and unlock new possibilities in biomedical research.
Product Name:
Human GAL4 (Galectin 4) CLIA Kit
SKU:
HUES00621
Target:
Human GAL4 (Galectin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL4. You can calculate the concentration of Human GAL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
93-110
84-100
Average (%)
93
100
91
1:4
Range (%)
94-112
98-114
100-111
Average (%)
102
106
105
1:8
Range (%)
91-106
102-117
94-107
Average (%)
98
108
102
1:16
Range (%)
95-112
99-111
93-105
Average (%)
103
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.11
625.53
1530.5
188.76
587.59
1435.41
Standard deviation
15.75
63.18
118.77
21.88
64.69
93.59
C V (%)
8.2
10.1
7.76
11.59
11.01
6.52
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL4 in samples. No significant cross-reactivity or interference between Human GAL4 and analogues was observed.
