The Human Gal8 (Galectin-8) CLIA Kit is a cutting-edge tool specifically created for the precise measurement of Galectin-8 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures dependable and reproducible results, making it perfect for a wide array of research purposes.Gal8, also known as Galectin-8, is a vital protein involved in various biological processes, including cell adhesion, signaling, and immune response regulation.
It plays a key role in diseases like inflammation, cancer, and autoimmune disorders, making it a crucial biomarker for investigating these conditions and developing potential treatment strategies.This revolutionary Gal8 CLIA Kit is an invaluable tool for researchers and clinicians seeking to delve deeper into the role of Galectin-8 in health and disease. Get your hands on this state-of-the-art kit now and unlock new possibilities in your research endeavors.
Product Name:
Human GAL8 (Galectin 8) CLIA Kit
SKU:
HUES00622
Target:
Human GAL8 (Galectin 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL8. You can calculate the concentration of Human GAL8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
91-105
95-109
Average (%)
97
98
103
1:4
Range (%)
101-117
90-105
84-98
Average (%)
107
98
90
1:8
Range (%)
90-105
93-107
92-104
Average (%)
96
99
97
1:16
Range (%)
100-116
104-117
88-100
Average (%)
107
110
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
89-103
94
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
191.34
463.25
1596.46
179.17
475.67
1504.06
Standard deviation
24.26
41.09
137.93
18.63
47.61
172.82
C V (%)
12.68
8.87
8.64
10.4
10.01
11.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL8 in samples. No significant cross-reactivity or interference between Human GAL8 and analogues was observed.
