The Human Galactosidase Alpha (GLA) ELISA Kit is a cutting-edge tool designed for the precise measurement of galactosidase alpha levels in human samples including serum, plasma, and cell culture supernatants. This innovative kit offers unparalleled sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research applications.Galactosidase alpha is a crucial enzyme involved in the breakdown of complex carbohydrates and lipids. Dysregulation of this enzyme has been linked to various metabolic disorders, including lysosomal storage diseases.
Therefore, accurate measurement of galactosidase alpha levels is essential for the study of these conditions and the development of potential therapeutic interventions.With its advanced technology and reliability, the Human GLA ELISA Kit is an invaluable tool for researchers seeking to explore the role of galactosidase alpha in health and disease. Trust in this kit to deliver precise and reproducible results, advancing your research efforts in the field of metabolic disorders.
Product Name:
Human Galactosidase Alpha (GLa) ELISA Kit
Product Code:
HUDL01194
Size:
96 Assays
Target:
GLa
Synonyms:
GALA, GL-A, Alpha-D-Galactoside Galactohydrolase, Alpha-D-Galactosidase A, Melibiase, Agalsidase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
