Human Gas6 (Growth Arrest Specific Protein 6) CLIA Kit
The Human Gas6 (Growth Arrest-Specific Protein 6) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Gas6 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Gas6 is a key protein involved in cell growth, survival, and regulation of inflammation. It plays a crucial role in various physiological processes, including immune response, cell adhesion, and apoptosis. Dysregulation of Gas6 has been implicated in various diseases, including cancer, cardiovascular diseases, and autoimmune disorders, highlighting its significance as a potential biomarker for disease progression and therapeutic targeting.
With its advanced technology and easy-to-use format, the Human Gas6 CLIA Kit offers researchers a valuable tool for studying the role of Gas6 in disease pathogenesis and identifying novel therapeutic strategies. Order yours today from Assay Genie and advance your research in the field of Gas6 biology.
Product Name:
Human Gas6 (Growth Arrest Specific Protein 6) CLIA Kit
SKU:
HUES00076
Target:
Human Gas6 (Growth Arrest Specific Protein 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Gas6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Gas6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Gas6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Gas6. You can calculate the concentration of Human Gas6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
87-100
91-103
Average (%)
100
92
97
1:4
Range (%)
103-117
98-113
93-106
Average (%)
109
104
98
1:8
Range (%)
95-109
96-110
100-115
Average (%)
102
101
105
1:16
Range (%)
90-105
96-108
95-108
Average (%)
97
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
98
EDTA plasma (n=5)
95-112
102
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.55
146.58
470.59
49.9
151.85
447.06
Standard deviation
6.44
12.86
48.09
6.18
17.42
37.55
C V (%)
13.0
8.77
10.22
12.38
11.47
8.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Gas6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Gas6 in samples. No significant cross-reactivity or interference between Human Gas6 and analogues was observed.
