Human Gas6 (Growth Arrest Specific Protein 6) ELISA Kit
The Gas6 (Growth Arrest Specific Protein 6) ELISA Kit is meticulously designed to quantitatively measure Gas6 levels in human biological samples.
Gas6, a crucial regulator of various cellular functions, plays a significant role in cell growth, survival, and immune response modulation. Accurate determination of Gas6 levels can provide valuable insights into its involvement in physiological processes and pathophysiological conditions. This ELISA kit is developed to provide exceptional sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures in place, the Gas6 ELISA Kit delivers robust performance and user-friendly operation, making it suitable for both research and clinical applications. Count on Assay Genie's Gas6 ELISA Kit for precise and reliable quantification of this essential biomarker in your investigations.
Product Name:
Human Gas6 (Growth Arrest Specific Protein 6) ELISA Kit
SKU:
AEES00051
Target:
Human Gas6 (Growth Arrest Specific Protein 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Gas6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Gas6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Gas6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Gas6. You can calculate the concentration of Human Gas6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-102
90-102
93-102
Average (%)
99
97
99
1:4
Range (%)
91-99
93-103
95-107
Average (%)
96
100
101
1:8
Range (%)
85-100
102-108
92-103
Average (%)
92
105
95
1:16
Range (%)
85-93
95-106
88-95
Average (%)
90
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
96
EDTA plasma (n=5)
95-106
98
Cell culture media (n=5)
92-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
186.58
462.83
1943.73
174.74
422.62
1682.57
Standard deviation
10.62
21.85
99.71
10.43
19.06
77.23
C V (%)
5.69
4.72
5.13
5.97
4.51
4.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Gas6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Gas6 in samples. No significant cross-reactivity or interference between Human Gas6 and analogues was observed.
