The Human GC (Glycocalicin) CLIA Kit is specifically developed for the precise measurement of glycocalicin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing reliable and consistent results for various research purposes.Glycocalicin is a key glycoprotein involved in cell adhesion and cell-cell interactions, playing a crucial role in various physiological and pathological processes.
Abnormal levels of glycocalicin have been linked to conditions like cancer, inflammatory diseases, and autoimmune disorders, showcasing its importance as a biomarker for investigating these conditions and potential therapeutic strategies.The Human GC (Glycocalicin) CLIA Kit is a valuable tool for researchers looking to study the role of glycocalicin in human health and disease, providing accurate and quantitative measurements to support their investigations.
Product Name:
Human GC (Glycocalicin) CLIA Kit
SKU:
HUES00279
Target:
Human GC (Glycocalicin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GC. You can calculate the concentration of Human GC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
93-107
94-108
Average (%)
98
100
102
1:4
Range (%)
91-106
100-115
88-101
Average (%)
97
108
93
1:8
Range (%)
98-112
97-112
101-117
Average (%)
106
105
107
1:16
Range (%)
92-105
86-99
83-96
Average (%)
97
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
244.84
439.44
2344.03
246.94
396.12
2198.03
Standard deviation
30.43
46.98
184.24
20.32
36.56
137.6
C V (%)
12.43
10.69
7.86
8.23
9.23
6.26
Sample type &Sample volume:
Plasma; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GC concentrations in Plasma.
Specificity:
This kit recognizes Human GC in samples. No significant cross-reactivity or interference between Human GC and analogues was observed.
