Human GCP-2 (Granulocyte Chemotactic Protein 2) CLIA Kit
The Human GCP-2 (Granulocyte Chemotactic Protein-2) CLIA Kit is specifically designed for the precise measurement of GCP-2 levels in human biological samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it an excellent choice for various research purposes.GCP-2, also known as CXCL6, is a key chemokine involved in the recruitment and activation of granulocytes, playing a crucial role in the inflammatory response. Dysregulation of GCP-2 levels has been implicated in various inflammatory diseases, including rheumatoid arthritis and inflammatory bowel disease, highlighting its importance as a biomarker for studying and developing treatments for these conditions.
With the Human GCP-2 CLIA Kit, researchers can accurately quantify GCP-2 levels in biological samples, allowing for a better understanding of its role in inflammation and potential therapeutic interventions. Trust in this kit for reliable and precise measurement of GCP-2 levels in your research studies.
Product Name:
Human GCP-2 (Granulocyte Chemotactic Protein 2) CLIA Kit
SKU:
HUES00046
Target:
Human GCP-2 (Granulocyte Chemotactic Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GCP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GCP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GCP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GCP-2. You can calculate the concentration of Human GCP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
96-113
92-103
Average (%)
101
103
97
1:4
Range (%)
90-103
94-110
99-116
Average (%)
96
102
106
1:8
Range (%)
103-118
100-114
100-118
Average (%)
109
106
108
1:16
Range (%)
99-117
95-110
98-113
Average (%)
108
101
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
88-103
96
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.25
109.38
412.58
54.03
117.58
449.6
Standard deviation
4.5
9.7
28.88
6.02
13.35
46.22
C V (%)
8.61
8.87
7.0
11.14
11.35
10.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GCP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GCP-2 in samples. No significant cross-reactivity or interference between Human GCP-2 and analogues was observed.
