Human GDF1 (Growth Differentiation Factor 1) CLIA Kit
The Human GDF1 (Growth Differentiation Factor 1) CLIA Kit is a cutting-edge assay designed for the precise measurement of GDF1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a wide range of research applications.GDF1 is a critical signaling molecule that plays a key role in cell differentiation and growth, particularly in embryonic development and tissue regeneration. Dysregulation of GDF1 signaling has been implicated in various diseases, including cancer, cardiovascular disorders, and developmental abnormalities.
Therefore, monitoring GDF1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its advanced technology and reliable performance, the Human GDF1 CLIA Kit is an indispensable tool for researchers studying the role of GDF1 in health and disease, offering a comprehensive solution for biomarker analysis and drug development efforts. Unlock the potential of GDF1 research with this innovative assay kit from Assay Genie.
Product Name:
Human GDF1 (Growth Differentiation Factor 1) CLIA Kit
SKU:
HUES01052
Target:
Human GDF1 (Growth Differentiation Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GDF1. You can calculate the concentration of Human GDF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
97-108
88-104
Average (%)
98
103
95
1:4
Range (%)
101-116
94-107
87-100
Average (%)
108
99
93
1:8
Range (%)
86-100
87-100
95-106
Average (%)
93
94
100
1:16
Range (%)
92-107
94-110
99-112
Average (%)
99
101
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-103
98
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.33
92.74
284.96
45.63
88.82
294.08
Standard deviation
5.41
8.85
26.62
5.67
8.85
30.55
C V (%)
12.2
9.54
9.34
12.43
9.96
10.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GDF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF1 in samples. No significant cross-reactivity or interference between Human GDF1 and analogues was observed.
