Human GDF2 (Growth Differentiation Factor 2) CLIA Kit
The Human GDF2 (Growth Differentiation Factor 2) CLIA Kit is specially designed for the precise measurement of GDF2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for various research applications.GDF2, also known as BMP9, is a critical factor in regulating cell growth, differentiation, and development. It plays a crucial role in bone formation, vascular development, and tissue regeneration.
Abnormal levels of GDF2 have been linked to various diseases, including cardiovascular disorders, skeletal defects, and cancer. Therefore, monitoring GDF2 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human GDF2 CLIA Kit from AssayGenie is an indispensable tool for researchers studying the role of GDF2 in health and disease, offering the accuracy and precision needed for reliable data analysis and interpretation.
Product Name:
Human GDF2 (Growth Differentiation Factor 2) CLIA Kit
SKU:
HUES01054
Target:
Human GDF2 (Growth Differentiation Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GDF2. You can calculate the concentration of Human GDF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
85-99
93-110
Average (%)
91
90
100
1:4
Range (%)
97-114
97-109
101-117
Average (%)
104
102
107
1:8
Range (%)
89-100
91-103
95-108
Average (%)
94
97
102
1:16
Range (%)
91-104
101-119
95-107
Average (%)
98
109
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
91-108
99
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
430.17
1170.66
3916.65
439.99
1204.4
3852.29
Standard deviation
39.06
121.75
266.72
56.85
87.68
367.12
C V (%)
9.08
10.4
6.81
12.92
7.28
9.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GDF2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF2 in samples. No significant cross-reactivity or interference between Human GDF2 and analogues was observed.
