Human GDF9 (Growth Differentiation Factor 9) CLIA Kit
The Human GDF9 (Growth Differentiation Factor 9) CLIA Kit is a reliable and sensitive assay for the precise measurement of GDF9 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it a valuable tool for researchers in a variety of fields.GDF9 is a key protein involved in regulating ovarian function and follicle development, making it a crucial biomarker for studying reproductive health and fertility.
Dysregulation of GDF9 has been linked to conditions such as polycystic ovary syndrome (PCOS) and infertility, highlighting the importance of accurately measuring GDF9 levels in research and clinical settings.With its high sensitivity and specificity, the Human GDF9 CLIA Kit offers a precise and reliable method for detecting GDF9 levels, providing valuable insights into reproductive health and potential therapeutic interventions.
Product Name:
Human GDF9 (Growth Differentiation Factor 9) CLIA Kit
SKU:
HUES01057
Target:
Human GDF9 (Growth Differentiation Factor 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GDF9. You can calculate the concentration of Human GDF9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
98-110
103-119
Average (%)
97
104
109
1:4
Range (%)
93-109
93-106
87-101
Average (%)
101
99
93
1:8
Range (%)
98-111
99-111
101-117
Average (%)
103
105
109
1:16
Range (%)
84-97
91-105
101-116
Average (%)
91
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-109
104
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.66
178.18
825.62
92.7
192.84
878.42
Standard deviation
9.51
13.47
60.35
8.29
17.22
100.05
C V (%)
9.45
7.56
7.31
8.94
8.93
11.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GDF9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF9 in samples. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
