Human GDP Dissociation Inhibitor 1 (GDI1) ELISA Kit
The Human GDP Dissociation Inhibitor 1 (GDI1) ELISA Kit from AssayGenie is a highly reliable and sensitive tool for detecting GDI1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high specificity, ensuring accurate and reproducible results for a variety of research applications.GDP Dissociation Inhibitor 1 (GDI1) is a critical protein involved in regulating the GDP-GTP exchange of small GTPases, which play key roles in cellular signaling and membrane trafficking processes. Dysregulation of GDI1 has been implicated in various diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance as a biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and easy-to-use format, the Human GDP Dissociation Inhibitor 1 (GDI1) ELISA Kit is a valuable tool for researchers looking to investigate the role of GDI1 in health and disease. Order yours today from AssayGenie and take your research to the next level.
Product Name:
Human GDP Dissociation Inhibitor 1 (GDI1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
