The Human GH2 (Growth Hormone 2) CLIA Kit is specifically designed for the precise measurement of growth hormone 2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it perfect for various research purposes.Growth hormone 2 is a critical hormone that plays a vital role in cell growth, metabolism, and tissue repair in the body.
It is essential for proper growth and development, making it a key biomarker for studying growth disorders, metabolic diseases, and other related conditions. The Human GH2 CLIA Kit provides researchers with a reliable tool to explore the role of growth hormone 2 in different physiological processes and diseases, paving the way for potential therapeutic advancements.
Product Name:
Human GH2 (Growth Hormone 2) CLIA Kit
SKU:
HUES01058
Target:
Human GH2 (Growth Hormone 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GH2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GH2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GH2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GH2. You can calculate the concentration of Human GH2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
89-105
97-110
Average (%)
106
96
103
1:4
Range (%)
95-110
95-108
101-116
Average (%)
101
100
109
1:8
Range (%)
100-112
85-99
95-106
Average (%)
106
91
100
1:16
Range (%)
93-103
87-100
88-101
Average (%)
98
94
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
105
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.42
114.82
356.43
49.24
120.04
346.59
Standard deviation
5.69
9.05
21.99
6.35
8.92
22.6
C V (%)
11.75
7.88
6.17
12.9
7.43
6.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GH2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GH2 in samples. No significant cross-reactivity or interference between Human GH2 and analogues was observed.
