Human GHRH (Growth Hormone Releasing Hormone) CLIA Kit
The Human GHRH (Growth Hormone Releasing Hormone) CLIA Kit is a cutting-edge diagnostic tool specifically designed for the precise and reliable detection of GHRH levels in human samples such as serum, plasma, and cell culture supernatants.This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications. GHRH is a vital hormone that plays a key role in regulating the release of growth hormone in the body, making it a crucial biomarker for various physiological processes.
Whether studying growth disorders, hormonal imbalances, or other related conditions, the Human GHRH CLIA Kit provides researchers with a powerful tool to investigate the intricate mechanisms of hormone regulation and develop potential therapeutic interventions. Trust in the accuracy and reliability of this innovative kit to advance your research and uncover new insights into the intricate world of hormone signaling.
Product Name:
Human GHRH (Growth Hormone Releasing Hormone) CLIA Kit
SKU:
HUES00667
Target:
Human GHRH (Growth Hormone Releasing Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GHRH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GHRH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GHRH. You can calculate the concentration of Human GHRH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
96-108
89-102
Average (%)
108
102
94
1:4
Range (%)
91-107
88-100
93-106
Average (%)
98
93
100
1:8
Range (%)
95-109
89-100
96-111
Average (%)
101
94
103
1:16
Range (%)
100-112
103-117
102-115
Average (%)
105
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
96-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.57
77.03
203.32
22.06
78.33
223.46
Standard deviation
2.19
6.59
22.2
2.61
9.25
24.85
C V (%)
9.29
8.56
10.92
11.83
11.81
11.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GHRH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GHRH in samples. No significant cross-reactivity or interference between Human GHRH and analogues was observed.
