Human GIP (Gastric Inhibitory Polypeptide) CLIA Kit
The Human GIP (Gastric Inhibitory Polypeptide) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GIP levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Gastric Inhibitory Polypeptide, also known as glucose-dependent insulinotropic polypeptide, plays a crucial role in regulating insulin secretion and glucose metabolism. Abnormal GIP levels have been associated with conditions such as diabetes and obesity, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With the Human GIP CLIA Kit, researchers can confidently measure GIP levels in biological samples, furthering our understanding of its role in health and disease. Whether investigating metabolic disorders or developing new treatments, this kit offers a powerful tool for cutting-edge research.
Product Name:
Human GIP (Gastric Inhibitory Polypeptide) CLIA Kit
SKU:
HUES01101
Target:
Human GIP (Gastric Inhibitory Polypeptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GIP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GIP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GIP. You can calculate the concentration of Human GIP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-111
90-102
99-115
Average (%)
101
96
106
1:4
Range (%)
102-114
95-110
101-119
Average (%)
108
101
108
1:8
Range (%)
99-113
100-113
103-121
Average (%)
105
107
110
1:16
Range (%)
92-104
90-106
104-118
Average (%)
99
97
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.11
134.14
359.89
53.27
124.8
371.65
Standard deviation
4.46
9.73
24.62
5.35
13.8
42.0
C V (%)
8.56
7.25
6.84
10.04
11.06
11.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GIP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GIP in samples. No significant cross-reactivity or interference between Human GIP and analogues was observed.
