Human GIP (Gastric Inhibitory Polypeptide) ELISA Kit
The Human GIP (Gastric Inhibitory Polypeptide) ELISA Kit is specifically designed for the precise measurement of GIP levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research applications.Gastric Inhibitory Polypeptide, or GIP, is a vital hormone that regulates insulin secretion and plays a key role in glucose metabolism.
Dysregulation of GIP levels has been implicated in metabolic disorders such as diabetes and obesity, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.This ELISA kit provides researchers with a reliable tool to quantify GIP levels, furthering our understanding of its biological functions and clinical relevance. Invest in the Human GIP ELISA Kit for precise and reliable measurements of GIP levels in your research studies.
Product Name:
Human GIP (Gastric Inhibitory Polypeptide) ELISA Kit
SKU:
HUES02978
Target:
Human GIP (Gastric Inhibitory Polypeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GIP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GIP. You can calculate the concentration of Human GIP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
88-102
90-106
Average (%)
101
94
97
1:4
Range (%)
91-104
85-98
87-102
Average (%)
96
91
94
1:8
Range (%)
89-105
80-93
85-99
Average (%)
96
86
90
1:16
Range (%)
93-107
85-97
88-101
Average (%)
99
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
277.55
484.96
1915.25
288.14
452.02
1992.73
Standard deviation
17.49
20.56
81.97
19.65
22.01
101.23
C V (%)
6.3
4.24
4.28
6.82
4.87
5.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GIP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GIP in samples. No significant cross-reactivity or interference between Human GIP and analogues was observed.
