Human GJ beta1 (Gap Junction Protein Beta 1) CLIA Kit
The Human GJB1 (Gap Junction Protein Beta 1) CLIA Kit is a high-quality assay designed for the precise measurement of GJB1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. GJB1, also known as connexin 32, is a key protein involved in gap junction communication between cells. Dysregulation of GJB1 has been linked to various neurological disorders, such as Charcot-Marie-Tooth disease, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its superior performance and ease of use, the Human GJB1 CLIA Kit is an essential tool for researchers investigating the role of GJB1 in health and disease. Get your kit today from Assay Genie and unlock new insights into cellular communication and disease mechanisms.
Product Name:
Human GJ beta1 (Gap Junction Protein Beta 1) CLIA Kit
SKU:
HUES00847
Target:
Human GJ beta1 (Gap Junction Protein Beta 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GJβ1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GJβ1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GJβ1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GJβ1. You can calculate the concentration of Human GJβ1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
90-103
87-100
Average (%)
106
97
93
1:4
Range (%)
90-103
101-114
91-103
Average (%)
97
108
97
1:8
Range (%)
101-117
102-114
95-106
Average (%)
109
108
101
1:16
Range (%)
94-104
93-109
96-110
Average (%)
99
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
91-101
96
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.14
533.3
1372.83
192.24
570.82
1434.8
Standard deviation
20.67
45.7
100.9
15.96
66.61
118.37
C V (%)
10.54
8.57
7.35
8.3
11.67
8.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GJβ1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GJβ1 in samples. No significant cross-reactivity or interference between Human GJβ1 and analogues was observed.
