The Human GKN1 (Gastrokine-1) CLIA Kit is specifically designed for the quantitative measurement of Gastrokine-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Gastrokine-1, also known as GKN1, is a critical protein involved in gastric mucosal protection and repair. It plays a vital role in maintaining the integrity of the gastric mucosa and has been implicated in various gastrointestinal disorders, including gastritis, peptic ulcers, and gastric cancer.
As such, Gastrokine-1 serves as an important biomarker for studying these conditions and exploring potential therapeutic interventions.The Human GKN1 CLIA Kit provided by Assay Genie offers researchers a reliable and efficient tool for measuring Gastrokine-1 levels in biological samples. With its high accuracy and precision, this kit is well-suited for advancing our understanding of gastric mucosal biology and developing novel strategies for managing gastrointestinal diseases.
Product Name:
Human GKN1 (Gastrokine 1) CLIA Kit
SKU:
HUES01097
Target:
Human GKN1 (Gastrokine 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GKN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GKN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GKN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GKN1. You can calculate the concentration of Human GKN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
100-114
92-109
Average (%)
106
108
99
1:4
Range (%)
86-100
93-108
98-115
Average (%)
93
99
105
1:8
Range (%)
89-104
94-107
84-98
Average (%)
95
100
91
1:16
Range (%)
94-112
101-118
92-107
Average (%)
102
108
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
95
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
92-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.8
195.06
755.2
111.99
207.88
690.43
Standard deviation
9.54
19.97
79.07
11.41
17.34
65.45
C V (%)
9.02
10.24
10.47
10.19
8.34
9.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GKN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GKN1 in samples. No significant cross-reactivity or interference between Human GKN1 and analogues was observed.
