The Human Glucosidase-Alpha ELISA Kit is specifically designed to accurately detect and quantify levels of glucosidase-alpha (GAA) in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.GAA is an important enzyme involved in the breakdown of complex sugars in the body. Dysregulation of GAA has been linked to various metabolic disorders, including Pompe disease, a rare genetic disorder characterized by the buildup of glycogen in cells.
Therefore, measuring GAA levels can provide valuable insights into potential disease mechanisms and aid in the development of targeted therapies.With its user-friendly design and reliable performance, the Human Glucosidase-Alpha ELISA Kit is an essential tool for researchers studying metabolic disorders and seeking to better understand the role of GAA in human health and disease. Order yours today from AssayGenie for precise and accurate quantitative analysis of GAA levels.
Product Name:
Human Glucosidase Alpha, Acid (GaA) ELISA Kit
Product Code:
HUDL01132
Size:
96 Assays
Target:
GaA
Synonyms:
LYAG, Acid Alpha-Glucosidase, Lysosomal Alpha-Glucosidase, Pompe Disease Glycogen Storage Disease Type II, Acid Maltase, Aglucosidase Alfa
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
