The Human GLUT4 (Glucose Transporter 4) CLIA Kit is a cutting-edge tool for accurately measuring GLUT4 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and consistent results, making it a valuable asset for various research applications.GLUT4 is a key protein responsible for regulating glucose uptake into cells, making it essential for maintaining proper blood sugar levels and overall metabolic health.
Dysregulation of GLUT4 is linked to conditions like diabetes and obesity, underscoring the importance of studying and understanding its function.This CLIA kit offers researchers a reliable and efficient way to quantitatively assess GLUT4 levels, providing valuable insights into various metabolic disorders and potential avenues for therapeutic intervention. Upgrade your research capabilities with the Human GLUT4 CLIA Kit today.
Product Name:
Human GLUT4 (Glucose Transporter 4) CLIA Kit
SKU:
HUES01011
Target:
Human GLUT4 (Glucose Transporter 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GLUT4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLUT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLUT4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GLUT4. You can calculate the concentration of Human GLUT4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
99-113
87-102
Average (%)
101
106
94
1:4
Range (%)
94-104
99-116
95-111
Average (%)
99
106
103
1:8
Range (%)
86-98
92-106
94-110
Average (%)
91
99
102
1:16
Range (%)
91-107
93-108
102-116
Average (%)
98
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
106
EDTA plasma (n=5)
93-106
98
Cell culture media (n=5)
97-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.23
344.02
1617.32
228.92
378.0
1640.37
Standard deviation
22.64
26.83
173.05
19.96
43.13
140.58
C V (%)
10.57
7.8
10.7
8.72
11.41
8.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GLUT4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLUT4 in samples. No significant cross-reactivity or interference between Human GLUT4 and analogues was observed.
