The Human Glycoprotein 39 (GP39) ELISA Kit is specifically designed for the precise measurement of GP39 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it a versatile tool for various research applications.GP39, also known as chondromodulin-I, is a glycoprotein primarily found in cartilage tissue where it plays a crucial role in maintaining cartilage integrity and promoting tissue regeneration.
Dysregulation of GP39 has been linked to conditions such as arthritis and joint disorders, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human GP39 ELISA Kit offers researchers a reliable and efficient method for analyzing GP39 levels in biological samples, facilitating further investigations into the role of this glycoprotein in cartilage health and disease pathology.
Product Name:
Human Glycoprotein 39, Cartilage (GP39) ELISA Kit
Product Code:
HUDL01225
Size:
96 Assays
Target:
GP39
Synonyms:
CHI3L1, CHI3-L1, YKL40, Chondrex, Chitinase 3-Like 1, Chondrocyte Protein YKL40, 39 kDa synovial protein
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
