Human Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) ELISA Kit
The Human Glycosylphosphatidylinositol-Anchored Molecule Like Protein (GML) ELISA Kit is specifically designed for the precise measurement of GML levels in human serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable tool for a variety of research purposes. GML is an important protein that is involved in various cellular functions, including cell adhesion and signaling.
Its dysregulation has been associated with certain diseases, making it a promising biomarker for understanding and potentially treating these conditions. By utilizing the Human GML ELISA Kit, researchers can gain valuable insights into the role of GML in health and disease, paving the way for new discoveries and therapeutic interventions. Order your kit today and unlock the potential of GML research.
Product Name:
Human Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) ELISA Kit
Product Code:
HUDL01213
Size:
96 Assays
Target:
GML
Synonyms:
LY6DL, GPI Anchored Molecule Like Protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
