The Human GM2 Ganglioside Activator (GM2A) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of GM2A levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for research applications in various fields.GM2 Ganglioside Activator (GM2A) plays a crucial role in lipid metabolism and lysosomal storage disorders, making it a valuable biomarker for studying conditions such as Tay-Sachs disease and Sandhoff disease.
By accurately measuring GM2A levels, researchers can better understand the mechanisms underlying these disorders and potentially develop new therapeutic interventions.Overall, the Human GM2 Ganglioside Activator (GM2A) ELISA Kit offers a reliable and efficient method for quantifying GM2A levels in human samples, making it an invaluable tool for researchers and clinicians working in the field of lysosomal storage disorders and related conditions.
Product Name:
Human GM2 Ganglioside Activator (GM2A) ELISA Kit
Product Code:
HUDL01211
Size:
96 Assays
Target:
GM2A
Synonyms:
SAP-3, Cerebroside Sulfate Activator Protein, Sphingolipid Activator Protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
