The Human GN Beta-1 (G Protein Beta 1) CLIA Kit is a powerful tool for accurately measuring levels of GN Beta-1 in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it a valuable asset for a variety of research applications.GN Beta-1 is a key protein involved in G protein signaling pathways, playing a crucial role in various cellular processes and signaling cascades. Dysregulation of GN Beta-1 has been linked to various diseases and disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
The Human GN Beta-1 CLIA Kit from Assay Genie provides researchers with a robust and efficient tool for quantifying GN Beta-1 levels, offering insights into its role in disease pathology and potential therapeutic targets. With its user-friendly design and precise measurement capabilities, this kit is an essential addition to any research laboratory focused on G protein signaling and related pathways.
Product Name:
Human GN beta1 (G Protein Beta 1) CLIA Kit
SKU:
HUES00531
Target:
Human GN beta1 (G Protein Beta 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GNβ1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GNβ1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GNβ1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GNβ1. You can calculate the concentration of Human GNβ1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-98
99-113
96-112
Average (%)
93
104
102
1:4
Range (%)
88-101
98-114
102-118
Average (%)
93
104
110
1:8
Range (%)
89-100
94-105
96-111
Average (%)
94
100
102
1:16
Range (%)
93-106
90-104
94-108
Average (%)
99
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
100-115
106
Cell culture media (n=5)
98-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.48
168.8
897.09
107.39
156.04
966.65
Standard deviation
12.39
18.38
70.78
13.5
16.23
75.88
C V (%)
12.21
10.89
7.89
12.57
10.4
7.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GNβ1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GNβ1 in samples. No significant cross-reactivity or interference between Human GNβ1 and analogues was observed.
