The Human GP-73 (Golgi Protein 73) CLIA Kit is a cutting-edge assay designed for the precise measurement of GP-73 levels in human serum, plasma, and cell culture supernatants. This kit boasts superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.GP-73 is a vital protein primarily found in the Golgi apparatus, playing a key role in cell transport and protein modification. Abnormal GP-73 levels have been linked to liver diseases, such as hepatocellular carcinoma and liver fibrosis, making it a valuable biomarker for studying these conditions and developing potential diagnostics and treatments.
The Human GP-73 CLIA Kit from Assaygenie offers researchers a reliable tool for investigating the role of GP-73 in liver diseases and exploring potential therapeutic interventions. Trust in the precision and accuracy of this kit to advance your research in the field of hepatology.
Product Name:
Human GP-73 (Golgi Protein 73) CLIA Kit
SKU:
HUES00759
Target:
Human GP-73 (Golgi Protein 73)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GP-73. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GP-73 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GP-73, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GP-73. You can calculate the concentration of Human GP-73 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
90-104
92-105
Average (%)
92
96
99
1:4
Range (%)
90-101
98-116
101-116
Average (%)
95
106
109
1:8
Range (%)
90-106
94-109
87-101
Average (%)
97
102
94
1:16
Range (%)
99-116
100-113
89-101
Average (%)
106
106
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
404.71
1191.61
2790.45
418.95
1241.67
2826.74
Standard deviation
47.88
93.78
236.63
43.57
119.82
209.46
C V (%)
11.83
7.87
8.48
10.4
9.65
7.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GP-73 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GP-73 in samples. No significant cross-reactivity or interference between Human GP-73 and analogues was observed.
