The Human GP5 (Glycoprotein V) Platelet CLIA Kit is specifically designed for the accurate detection of GP5 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GP5, also known as Glycoprotein V, is a platelet surface glycoprotein that plays a critical role in platelet adhesion and aggregation. Dysregulation of GP5 expression has been linked to various platelet disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Human GP5 Platelet CLIA Kit provides researchers with a reliable tool for investigating the role of GP5 in platelet function and its implications for various pathologies. Its ease of use and high performance make it a valuable addition to any laboratory seeking to advance their understanding of platelet biology and related diseases.
Product Name:
Human GP5 (Glycoprotein V, Platelet) CLIA Kit
SKU:
HUES01078
Target:
Human GP5 (Glycoprotein V, Platelet)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GP5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GP5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GP5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GP5. You can calculate the concentration of Human GP5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
87-100
101-116
Average (%)
109
94
107
1:4
Range (%)
92-102
101-119
91-106
Average (%)
97
109
97
1:8
Range (%)
94-111
96-112
86-100
Average (%)
102
104
91
1:16
Range (%)
84-96
87-99
96-110
Average (%)
91
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
90
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
1.68
9.11
1.07
1.79
8.62
Standard deviation
0.08
0.15
0.82
0.13
0.18
0.66
C V (%)
8.16
8.93
9.0
12.15
10.06
7.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GP5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GP5 in samples. No significant cross-reactivity or interference between Human GP5 and analogues was observed.
