The Human GP6 (Glycoprotein VI) Platelet CLIA Kit is a cutting-edge assay designed for the precise measurement of GP6 levels in human plasma, serum, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.GP6, also known as Glycoprotein VI, is a critical component involved in platelet activation and adhesion, playing a crucial role in hemostasis and thrombosis.
Dysregulation of GP6 has been linked to various cardiovascular disorders, making it a valuable biomarker for exploring these conditions and potential therapeutic interventions.With its user-friendly format and reliable performance, the Human GP6 Platelet CLIA Kit is an indispensable tool for researchers seeking to delve deeper into the intricate mechanisms of platelet function and their implications in health and disease.
Product Name:
Human GP6 (Glycoprotein VI, Platelet) CLIA Kit
SKU:
HUES01079
Target:
Human GP6 (Glycoprotein VI, Platelet)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GP6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GP6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GP6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GP6. You can calculate the concentration of Human GP6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
91-105
93-106
Average (%)
97
97
100
1:4
Range (%)
99-114
90-100
84-96
Average (%)
105
95
91
1:8
Range (%)
93-108
85-97
96-113
Average (%)
100
91
103
1:16
Range (%)
93-107
99-115
89-103
Average (%)
98
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.05
4.57
16.89
1.85
4.19
15.73
Standard deviation
0.22
0.4
1.78
0.19
0.39
1.37
C V (%)
10.73
8.75
10.54
10.27
9.31
8.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GP6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GP6 in samples. No significant cross-reactivity or interference between Human GP6 and analogues was observed.
