The Human GPC2 (Glypican 2) CLIA Kit is a cutting-edge assay designed for the precise measurement of Glypican 2 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an essential tool for various research applications.Glypican 2 is a key protein that plays a crucial role in various biological processes, including cell growth, differentiation, and development.
Its dysregulation has been linked to several diseases, such as cancer and neurological disorders, highlighting its potential as a valuable biomarker for understanding these conditions and developing targeted interventions.Get reliable and insightful data on Glypican 2 levels with the Human GPC2 (Glypican 2) CLIA Kit, empowering your research and contributing to advancements in the field of biomarker discovery and therapeutic development.
Product Name:
Human GPC2 (Glypican 2) CLIA Kit
SKU:
HUES00956
Target:
Human GPC2 (Glypican 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPC2. You can calculate the concentration of Human GPC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
102-115
103-118
Average (%)
93
109
108
1:4
Range (%)
100-118
87-103
98-111
Average (%)
107
94
105
1:8
Range (%)
88-102
86-98
96-111
Average (%)
95
92
103
1:16
Range (%)
100-115
101-116
89-100
Average (%)
107
108
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.72
299.39
752.57
106.53
273.37
749.76
Standard deviation
12.42
31.08
66.68
13.26
26.76
55.26
C V (%)
11.42
10.38
8.86
12.45
9.79
7.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPC2 in samples. No significant cross-reactivity or interference between Human GPC2 and analogues was observed.
