The Human GPC3 (Glypican 3) CLIA Kit is a cutting-edge assay designed for the accurate detection of Glypican 3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Glypican 3 is a key protein involved in cell proliferation, growth regulation, and development. Abnormal expression of Glypican 3 has been linked to various diseases, including hepatocellular carcinoma and other cancers.
As such, this kit is essential for researchers studying these conditions and working towards the development of potential therapies.With its advanced technology and reliable performance, the Human GPC3 CLIA Kit is a valuable tool for researchers looking to delve deeper into the role of Glypican 3 in health and disease.
Product Name:
Human GPC3 (Glypican 3) CLIA Kit
SKU:
HUES00957
Target:
Human GPC3 (Glypican 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPC3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPC3. You can calculate the concentration of Human GPC3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
94-110
95-112
Average (%)
91
100
103
1:4
Range (%)
92-107
92-105
85-97
Average (%)
98
98
91
1:8
Range (%)
88-101
91-103
89-105
Average (%)
94
98
96
1:16
Range (%)
88-102
100-117
86-101
Average (%)
94
107
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
100-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.97
275.43
862.58
111.4
250.53
948.6
Standard deviation
13.86
23.93
63.31
11.53
27.83
108.52
C V (%)
12.6
8.69
7.34
10.35
11.11
11.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPC3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPC3 in samples. No significant cross-reactivity or interference between Human GPC3 and analogues was observed.
