Human GPI (Glucose 6 Phosphate Isomerase) CLIA Kit
The Human GPI (Glucose-6-Phosphate Isomerase) CLIA Kit is designed for the accurate detection of GPI levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.GPI is a key enzyme involved in glycolysis, playing a crucial role in the interconversion of glucose-6-phosphate and fructose-6-phosphate.
Dysregulation of GPI has been associated with various diseases, including cancer, autoimmune diseases, and metabolic disorders. Therefore, the Human GPI CLIA Kit serves as an essential tool for studying these conditions and exploring potential therapeutic interventions.
Product Name:
Human GPI (Glucose 6 Phosphate Isomerase) CLIA Kit
SKU:
HUES01006
Target:
Human GPI (Glucose 6 Phosphate Isomerase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPI. You can calculate the concentration of Human GPI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
101-117
83-96
Average (%)
100
108
90
1:4
Range (%)
85-95
91-104
99-114
Average (%)
90
98
107
1:8
Range (%)
100-115
98-112
94-106
Average (%)
109
105
99
1:16
Range (%)
102-117
93-106
89-104
Average (%)
107
99
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
97-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.63
100.91
367.25
50.25
92.18
370.12
Standard deviation
4.7
10.77
37.83
5.41
6.45
22.39
C V (%)
9.47
10.67
10.3
10.77
7.0
6.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPI in samples. No significant cross-reactivity or interference between Human GPI and analogues was observed.
