Human GPR120 (G Protein Coupled Receptor 120) CLIA Kit
The Human GPR120 (G-Protein Coupled Receptor 120) CLIA Kit is a cutting-edge assay designed for the accurate detection of GPR120 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GPR120 is a key receptor involved in lipid metabolism and regulation of inflammation, playing a crucial role in conditions such as obesity, diabetes, and inflammatory disorders.
By accurately measuring GPR120 levels, researchers can gain valuable insights into these conditions and develop potential therapeutic interventions.Overall, the Human GPR120 CLIA Kit provides researchers with a powerful tool for studying the role of GPR120 in various physiological and pathological processes, ultimately leading to advancements in the understanding and treatment of related diseases.
Product Name:
Human GPR120 (G Protein Coupled Receptor 120) CLIA Kit
SKU:
HUES00736
Target:
Human GPR120 (G Protein Coupled Receptor 120)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPR120. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPR120 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPR120, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPR120. You can calculate the concentration of Human GPR120 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
100-117
97-111
Average (%)
103
107
104
1:4
Range (%)
97-115
96-111
97-111
Average (%)
105
102
103
1:8
Range (%)
95-107
99-114
97-108
Average (%)
101
108
103
1:16
Range (%)
95-107
88-98
98-114
Average (%)
101
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
108
EDTA plasma (n=5)
98-110
105
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.03
4.78
0.44
1.09
4.4
Standard deviation
0.06
0.11
0.37
0.05
0.09
0.31
C V (%)
12.5
10.68
7.74
11.36
8.26
7.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPR120 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPR120 in samples. No significant cross-reactivity or interference between Human GPR120 and analogues was observed.
