Description
| Product Name: | Nanodisc Human GPR31 Protein |
| SKU: | HDFP294 |
| Size: | 10μg |
| Target: | GPR31 |
| Tag: | C-Flag Tag |
| Expression Host: | HEK293 |
| Conjugate: | Unconjugated |
| Synonyms: | 12-HETER, HETER, HETER1 |
| Protein Families: | GPCR, Transmembrane, Druggable Genome. |
| Protein Description: | Human GPR31 full length protein-synthetic nanodisc |
| Protein Pathways: | GPCRDB Class A Rhodopsin-like, Chemokines, Chemokine and Receptor. |
| Molecular Weight: | The human full length GPR31 protein has a MW of 35.1kDa |
High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.[UniProtKB/Swiss-Prot Function]
| Uniprot ID: | O00270 |
| Formulation & Reconstitution: | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping: | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Usage: | Research use only |
