Human GPRC5A (G Protein Coupled Receptor, Family C, Group 5, Member A) CLIA Kit
The Human GPRC5A (G Protein-Coupled Receptor Family C Group 5 Member A) CLIA Kit is specifically designed for the quantitative measurement of GPRC5A levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.GPRC5A is a member of the G protein-coupled receptor family and plays a critical role in various cellular processes such as cell signaling, proliferation, and differentiation.
Dysregulation of GPRC5A has been linked to the development and progression of various diseases, including cancer and metabolic disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human GPRC5A CLIA Kit provides researchers with a reliable tool for exploring the role of GPRC5A in human health and disease, ultimately advancing our understanding of cellular signaling pathways and potential treatment strategies.
Product Name:
Human GPRC5A (G Protein Coupled Receptor, Family C, Group 5, Member A) CLIA Kit
SKU:
HUES00637
Target:
Human GPRC5A (G Protein Coupled Receptor, Family C, Group 5, Member A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPRC5A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPRC5A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPRC5A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPRC5A. You can calculate the concentration of Human GPRC5A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
84-96
97-114
Average (%)
94
91
105
1:4
Range (%)
94-109
100-114
93-106
Average (%)
99
107
100
1:8
Range (%)
97-112
90-106
102-116
Average (%)
103
97
110
1:16
Range (%)
102-116
96-111
101-118
Average (%)
109
103
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
84-96
90
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.24
419.4
1399.72
188.31
452.08
1309.06
Standard deviation
21.33
31.71
93.22
15.35
48.55
125.8
C V (%)
11.33
7.56
6.66
8.15
10.74
9.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPRC5A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPRC5A in samples. No significant cross-reactivity or interference between Human GPRC5A and analogues was observed.
