Human GPS1 (G Protein Pathway Suppressor 1) ELISA Kit
The Human GPS1 (G-Protein Pathway Suppressor 1) ELISA Kit is specifically designed for the accurate detection of GPS1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it an invaluable tool for various research applications.GPS1 is a key protein involved in the suppression of G-protein signaling pathways, regulating various cellular processes. Dysregulation of GPS1 has been linked to numerous diseases, including cancer, cardiovascular diseases, and immune disorders, highlighting its importance as a potential therapeutic target.
With the Human GPS1 ELISA Kit, researchers can easily measure GPS1 levels in biological samples, allowing for a better understanding of its role in disease development and progression. This kit provides a reliable and efficient way to study GPS1 and its involvement in various cellular pathways, paving the way for new insights and potential treatment options.
Product Name:
Human GPS1 (G Protein Pathway Suppressor 1) ELISA Kit
SKU:
HUES03333
Target:
Human GPS1 (G Protein Pathway Suppressor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
46.88 ng/mL
Detection range:
78.13-5000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GPS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GPS1. You can calculate the concentration of Human GPS1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
86-101
85-96
Average (%)
102
92
90
1:4
Range (%)
87-99
85-97
88-99
Average (%)
93
90
93
1:8
Range (%)
88-103
85-99
83-94
Average (%)
96
90
89
1:16
Range (%)
91-102
85-98
84-96
Average (%)
96
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
89-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
269.93
606.86
2458.91
255.51
664.56
2396.01
Standard deviation
17.73
28.58
82.62
15.51
36.28
100.63
C V (%)
6.57
4.71
3.36
6.07
5.46
4.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GPS1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPS1 in samples. No significant cross-reactivity or interference between Human GPS1 and analogues was observed.
