Human GR alpha (Glucocorticoid Receptor Alpha) CLIA Kit
The Human GR (Glucocorticoid Receptor) Alpha CLIA Kit is specifically designed for the quantitative measurement of GR alpha levels in human samples. This kit offers high sensitivity and accuracy, allowing for precise and reliable results in serum, plasma, and cell culture supernatants.The glucocorticoid receptor alpha plays a critical role in mediating the biological effects of glucocorticoids, which are important hormones involved in various physiological processes, including metabolism, immune response, and stress regulation.
Dysregulation of GR alpha has been associated with a variety of diseases, such as immune disorders, inflammatory conditions, and metabolic disorders.With the Human GR Alpha CLIA Kit, researchers can easily assess GR alpha levels in human samples to further understand its role in health and disease, paving the way for new insights and potential therapeutic strategies.
Product Name:
Human GR alpha (Glucocorticoid Receptor Alpha) CLIA Kit
SKU:
HUES01082
Target:
Human GR alpha (Glucocorticoid Receptor Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GRα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GRα. You can calculate the concentration of Human GRα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
97-111
91-104
Average (%)
95
103
96
1:4
Range (%)
96-112
96-111
95-111
Average (%)
102
104
101
1:8
Range (%)
84-100
98-112
87-100
Average (%)
91
104
93
1:16
Range (%)
100-114
87-98
90-106
Average (%)
105
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
90-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.81
562.62
1478.9
179.91
508.4
1405.65
Standard deviation
21.88
46.13
125.56
16.98
45.65
158.28
C V (%)
11.35
8.2
8.49
9.44
8.98
11.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GRα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GRα in samples. No significant cross-reactivity or interference between Human GRα and analogues was observed.
