Human GR beta (Glucocorticoid Receptor Beta) CLIA Kit
The Human GR-beta (Glucocorticoid Receptor beta) CLIA Kit is specifically designed to accurately measure glucocorticoid receptor beta levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.The glucocorticoid receptor beta is a key component in the regulation of cellular response to glucocorticoid hormones, playing a crucial role in immune response, metabolism, and stress regulation.
Dysregulation of glucocorticoid receptor beta has been associated with various diseases such as arthritis, asthma, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With its reliable performance and broad applicability, the Human GR-beta CLIA Kit is an essential tool for researchers looking to investigate the role of glucocorticoid receptor beta in health and disease.
Product Name:
Human GR beta (Glucocorticoid Receptor Beta) CLIA Kit
SKU:
HUES01083
Target:
Human GR beta (Glucocorticoid Receptor Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GRβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GRβ. You can calculate the concentration of Human GRβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
89-102
92-106
Average (%)
102
95
98
1:4
Range (%)
89-100
104-118
88-100
Average (%)
94
110
94
1:8
Range (%)
93-104
93-106
99-113
Average (%)
98
99
105
1:16
Range (%)
96-110
86-96
92-107
Average (%)
102
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
98
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
99-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.72
137.47
461.05
50.07
134.67
433.22
Standard deviation
5.84
14.87
45.37
6.22
14.18
33.83
C V (%)
11.51
10.82
9.84
12.42
10.53
7.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GRβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GRβ in samples. No significant cross-reactivity or interference between Human GRβ and analogues was observed.
