Human Grb10 (Growth Factor Receptor Bound Protein 10) CLIA Kit
The Human GRB10 (Growth Factor Receptor Bound Protein 10) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GRB10 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an invaluable tool for a variety of research applications.GRB10 is a vital protein involved in growth factor signaling pathways, influencing cell growth, proliferation, and differentiation.
Dysregulation of GRB10 has been implicated in various diseases, including cancer, diabetes, and obesity, making it a crucial biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human GRB10 CLIA Kit offers researchers a powerful tool for studying the role of GRB10 in disease pathology and exploring its potential as a therapeutic target.
Product Name:
Human Grb10 (Growth Factor Receptor Bound Protein 10) CLIA Kit
SKU:
HUES01062
Target:
Human Grb10 (Growth Factor Receptor Bound Protein 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Grb10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Grb10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Grb10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Grb10. You can calculate the concentration of Human Grb10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
91-104
93-109
Average (%)
108
98
99
1:4
Range (%)
98-110
89-100
100-114
Average (%)
104
95
105
1:8
Range (%)
87-103
88-100
100-116
Average (%)
94
94
106
1:16
Range (%)
96-114
90-100
84-98
Average (%)
104
95
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
97-109
102
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.78
562.61
1937.24
196.12
605.91
1894.66
Standard deviation
20.86
57.05
153.43
18.12
50.41
126.37
C V (%)
10.6
10.14
7.92
9.24
8.32
6.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Grb10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Grb10 in samples. No significant cross-reactivity or interference between Human Grb10 and analogues was observed.
