Human Grb14 (Growth Factor Receptor Bound Protein 14) ELISA Kit
The Human GRB14 (Growth Factor Receptor-Bound Protein 14) ELISA Kit is specially designed for the precise measurement of GRB14 levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for various research applications.GRB14 is a key protein that interacts with growth factor receptors, modulating signaling pathways involved in cell growth and proliferation.
Dysregulation of GRB14 has been linked to diseases such as diabetes, obesity, and cancer, highlighting its significance as a potential therapeutic target and biomarker for disease progression.This ELISA kit provides researchers with a valuable tool for studying the role of GRB14 in health and disease, aiding in the development of novel treatments and diagnostic strategies. Trust in the reliability and precision of the Human GRB14 ELISA Kit for your research needs.
Product Name:
Human Grb14 (Growth Factor Receptor Bound Protein 14) ELISA Kit
SKU:
HUES02872
Target:
Human Grb14 (Growth Factor Receptor Bound Protein 14)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Grb14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Grb14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Grb14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Grb14. You can calculate the concentration of Human Grb14 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
93-111
90-103
Average (%)
99
101
95
1:4
Range (%)
95-107
82-95
83-96
Average (%)
100
89
89
1:8
Range (%)
87-101
84-97
84-94
Average (%)
93
89
89
1:16
Range (%)
91-105
83-95
89-100
Average (%)
97
88
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
97
EDTA plasma (n=5)
86-103
94
Cell culture media (n=5)
96-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.05
4.3
0.45
1.01
4.08
Standard deviation
0.03
0.05
0.15
0.03
0.04
0.17
C V (%)
6.38
4.76
3.49
6.67
3.96
4.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Grb14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Grb14 in samples. No significant cross-reactivity or interference between Human Grb14 and analogues was observed.
