Human Grb2 (Growth Factor Receptor Bound Protein 2) CLIA Kit
The Human GRB2 (Growth Factor Receptor-Bound Protein 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GRB2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for a wide range of research applications.GRB2 is a critical adapter protein involved in cellular signaling pathways, particularly in growth factor receptor signaling.
Dysregulation of GRB2 has been implicated in various diseases, including cancer, diabetes, and autoimmune disorders, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.With its high sensitivity and specificity, the Human GRB2 CLIA Kit from AssayGenie offers researchers a powerful tool for studying the role of GRB2 in health and disease, paving the way for new insights and potential therapeutic interventions.
Product Name:
Human Grb2 (Growth Factor Receptor Bound Protein 2) CLIA Kit
SKU:
HUES01063
Target:
Human Grb2 (Growth Factor Receptor Bound Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Grb2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Grb2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Grb2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Grb2. You can calculate the concentration of Human Grb2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
87-98
102-118
Average (%)
103
92
108
1:4
Range (%)
91-102
91-105
87-103
Average (%)
96
99
94
1:8
Range (%)
92-102
89-101
85-98
Average (%)
97
95
92
1:16
Range (%)
85-97
87-103
86-100
Average (%)
90
94
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
97-110
102
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.06
321.08
693.98
95.44
350.05
692.91
Standard deviation
8.57
27.29
76.34
11.53
39.24
46.42
C V (%)
9.02
8.5
11.0
12.08
11.21
6.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Grb2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Grb2 in samples. No significant cross-reactivity or interference between Human Grb2 and analogues was observed.
