Human GROgamma (Growth Regulated Oncogene Gamma) CLIA Kit
The Human GROgamma (Growth Regulated Oncogene-Gamma) CLIA Kit is a highly sensitive assay designed for the accurate detection of GROgamma levels in human samples, including serum, plasma, and cell culture supernatants. With its high specificity and reproducibility, this kit provides reliable results for a variety of research applications.GROgamma is a key protein involved in inflammatory responses and cell migration, playing a critical role in various diseases such as cancer and inflammation.
By measuring GROgamma levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Human GROgamma CLIA Kit is an essential tool for studying the role of GROgamma in disease development and progression, enabling researchers to advance our understanding of these complex biological processes.
Product Name:
Human GROgamma (Growth Regulated Oncogene Gamma) CLIA Kit
SKU:
HUES01051
Target:
Human GROgamma (Growth Regulated Oncogene Gamma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GROγ/CXCL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GROγ/CXCL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROγ/CXCL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GROγ/CXCL3. You can calculate the concentration of Human GROγ/CXCL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
87-100
91-103
Average (%)
95
92
96
1:4
Range (%)
93-108
90-102
100-119
Average (%)
100
97
109
1:8
Range (%)
101-115
91-103
97-108
Average (%)
108
98
102
1:16
Range (%)
96-111
95-110
92-103
Average (%)
103
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
105
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.83
37.48
198.26
21.76
36.53
199.46
Standard deviation
1.98
4.03
16.26
1.79
4.06
17.47
C V (%)
9.07
10.75
8.2
8.23
11.11
8.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GROγ/CXCL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GROγ/CXCL3 in samples. No significant cross-reactivity or interference between Human GROγ/CXCL3 and analogues was observed.
