Human Growth Arrest Specific Protein 6 (GAS6) ELISA Kit
The Human Growth Arrest-Specific Protein 6 (GAS6) ELISA Kit is specifically designed for the precise detection of GAS6 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures dependable and consistent results, making it an excellent choice for a variety of research applications. GAS6 is a vital protein involved in cell growth and survival, as well as regulating immune responses and inflammation. It plays a crucial role in various physiological processes and is implicated in diseases such as cancer, autoimmune disorders, and cardiovascular diseases.
Therefore, studying GAS6 levels can provide valuable insights into these conditions and aid in the development of potential treatments. With the Human Growth Arrest-Specific Protein 6 (GAS6) ELISA Kit, researchers can accurately measure GAS6 levels in biological samples, advancing their understanding of the role of this protein in health and disease.
Product Name:
Human Growth Arrest Specific Protein 6 (GAS6) ELISA Kit
Product Code:
HUDL01154
Size:
96 Assays
Target:
GAS6
Synonyms:
AXLLG, AXSF, AXL Stimulatory Factor, AXL receptor tyrosine kinase ligand
Detection Method:
Sandwich
Range:
6.25-400pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
