Human Growth Differentiation Factor 1 (GDF1) ELISA Kit
The Human Growth Differentiation Factor 1 (GDF1) ELISA Kit is a highly reliable and sensitive tool for the quantitative detection of GDF1 levels in human serum, plasma, and cell culture supernatants. This kit is designed to provide accurate and reproducible results, making it ideal for a diverse range of research applications.GDF1 is a key protein involved in various cellular processes including growth, differentiation, and development. It plays a crucial role in maintaining tissue homeostasis and regulating cell function.
Dysregulation of GDF1 has been linked to various diseases such as cardiovascular disorders, skeletal abnormalities, and neurodevelopmental conditions.By using the GDF1 ELISA Kit, researchers can gain valuable insights into the role of GDF1 in physiological and pathological conditions, paving the way for potential therapeutic interventions and disease management strategies. Don't miss out on this essential tool for your research needs!
Product Name:
Human Growth Differentiation Factor 1 (GDF1) ELISA Kit
Product Code:
HUDL01167
Size:
96 Assays
Target:
GDF1
Synonyms:
Embryonic growth/differentiation factor 1
Detection Method:
Sandwich
Range:
31.2-2,000 pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
