The Human GS (Gelsolin) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of gelsolin levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and consistent results, making it suitable for various research applications.Gelsolin is an important protein involved in regulating actin dynamics and cell migration, playing a crucial role in processes such as cell motility and cytoskeleton organization.
Dysregulation of gelsolin has been linked to various diseases, including cancer, Alzheimer's disease, and inflammatory conditions, highlighting its significance as a potential biomarker for disease progression and treatment monitoring.The Human GS (Gelsolin) CLIA Kit offers researchers a valuable tool for studying the function and regulation of gelsolin in health and disease, providing insights into its potential therapeutic applications in various medical conditions.
Product Name:
Human GS (Gelsolin) CLIA Kit
SKU:
HUES00993
Target:
Human GS (Gelsolin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GS. You can calculate the concentration of Human GS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
86-100
88-104
Average (%)
102
93
95
1:4
Range (%)
85-99
88-100
101-115
Average (%)
91
93
107
1:8
Range (%)
97-113
83-97
96-109
Average (%)
104
90
102
1:16
Range (%)
95-108
96-110
91-107
Average (%)
101
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.1
11.97
32.19
4.09
12.54
30.78
Standard deviation
0.42
1.1
3.47
0.37
1.01
2.63
C V (%)
10.24
9.19
10.78
9.05
8.05
8.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GS in samples. No significant cross-reactivity or interference between Human GS and analogues was observed.
