Human GSTpi (Glutathione S Transferases Pi) CLIA Kit
The Human GSTPi (Glutathione S-Transferase Pi) CLIA Kit is a specialized assay designed for the accurate measurement of GSTPi levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.GSTPi is an important enzyme involved in detoxification processes, playing a key role in protecting cells from oxidative stress and harmful chemicals. Abnormal levels of GSTPi have been associated with various diseases, including cancer, liver diseases, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and ease of use, the Human GSTPi CLIA Kit is an essential tool for researchers looking to study the role of GSTPi in health and disease, providing valuable insights into cellular detoxification mechanisms and potential treatment strategies.
Product Name:
Human GSTpi (Glutathione S Transferases Pi) CLIA Kit
SKU:
HUES00777
Target:
Human GSTpi (Glutathione S Transferases Pi)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GSTpi. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GSTpi and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GSTpi, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GSTpi. You can calculate the concentration of Human GSTpi in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
101-117
94-110
Average (%)
93
107
101
1:4
Range (%)
96-110
94-106
85-100
Average (%)
103
101
91
1:8
Range (%)
83-95
85-99
98-115
Average (%)
90
92
105
1:16
Range (%)
87-97
95-108
87-102
Average (%)
92
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
95
EDTA plasma (n=5)
100-111
105
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.62
3.83
12.77
1.5
4.0
12.01
Standard deviation
0.18
0.44
1.36
0.18
0.4
1.29
C V (%)
11.11
11.49
10.65
12.0
10.0
10.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GSTpi concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GSTpi in samples. No significant cross-reactivity or interference between Human GSTpi and analogues was observed.
