Human Guanylate Cyclase Activator 2B (GUCA2B) ELISA Kit
The Human Guanylate Cyclase Activator 2B (GUCA2B) ELISA Kit is specifically designed for the accurate detection of GUCA2B levels in human serum, plasma, and cell culture supernatants. This kit ensures high sensitivity and specificity, guaranteeing reliable and reproducible results for a variety of research applications.GUCA2B is known to be a key activator of guanylate cyclase, playing a crucial role in the regulation of intestinal fluid and electrolyte transport.
Dysregulation of GUCA2B has been linked to conditions such as gastrointestinal disorders, making it an important biomarker for studying these diseases and potentially developing targeted therapies.With its advanced technology and proven accuracy, the Human GUCA2B ELISA Kit is a valuable tool for researchers investigating the role of GUCA2B in health and disease. Order now and take your research to the next level.
Product Name:
Human Guanylate Cyclase Activator 2B (GUCA2B) ELISA Kit
Product Code:
HUDL01284
Size:
96 Assays
Target:
GUCA2B
Synonyms:
UGN, GCAP-II, Uroguanylin, Guanylate cyclase C-activating peptide II
Detection Method:
Sandwich
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
