The Human H-Cadherin (Cadherin Heart) CLIA Kit is a specialized assay designed for the accurate measurement of H-Cadherin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential tool for researchers in various fields.H-Cadherin, also known as Cadherin Heart, is a key protein involved in cell adhesion and tissue organization, particularly in the cardiovascular system. Dysregulation of H-Cadherin expression has been implicated in various heart diseases and can serve as a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human H-Cadherin CLIA Kit, researchers can accurately measure H-Cadherin levels in human samples, allowing for a deeper understanding of its role in cardiovascular health and disease. This kit is easy to use and provides precise results, making it an indispensable tool for studying H-Cadherin in research settings.
Product Name:
Human H-Cadherin (Cadherin, Heart) CLIA Kit
SKU:
HUES00533
Target:
Human H-Cadherin (Cadherin, Heart)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human H-Cadherin. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human H-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human H-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human H-Cadherin. You can calculate the concentration of Human H-Cadherin in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
102-116
92-106
Average (%)
93
110
98
1:4
Range (%)
98-113
98-115
89-103
Average (%)
105
105
96
1:8
Range (%)
94-108
86-101
102-114
Average (%)
102
93
108
1:16
Range (%)
86-101
89-103
94-107
Average (%)
93
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.47
251.28
887.18
107.43
248.23
974.77
Standard deviation
12.39
24.65
97.06
11.18
23.51
62.48
C V (%)
12.46
9.81
10.94
10.41
9.47
6.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human H-Cadherin concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human H-Cadherin in samples. No significant cross-reactivity or interference between Human H-Cadherin and analogues was observed.
